Intro to flow video from Coulter: https://www.beckman.com/resources/videos/scientific/introduction-to-flow-cytometry
Introduction to Flow Cytometry – BC-1424VID05.16-A
| Cell Type | CD Marker |
| Leukocyte | CD45 |
| T cells (all) | CD3 |
| T helper cells | CD4 |
| Cytotoxic T cells | C8D |
| B cells | CD19 and CD20 |
| Myeloid | CD13, CD33, CD15, CD 117 |
| T-Cell | CD2, CD3, CD5, CD7 |
| B-Cell | CD19, CD20, CD22, CD79a |
| Megakaryoblastic | CD41, CD61 |
CD138 – multiple myeloma
CD52 – lymphocytes, monocytes, eosinophils, thymocytes, and macrophages
- 2 T-Cell
- 3 all T-cells
- 4 helper T cells
- 5 T- Cell
- 7 T-Cell
- 8 cytotoxic T-Cell
- 9 platelet activation (HIV entry)
- 10 lung intestine breast carcinoma
- 11 leukocyte adhesion
- 13 Myeloid
- 14 monocyte
- 15 Myeloid
- 16 natural killers
- 19 B cell juvenile
- 20 B cell mature
- 22 B cell
- 23 IgE receptor
- 25 developed t cell
- 27 deficiency associated with SCID
- 33 – for AML treatment
- 34 – most blasts
- 38
- 45 – all luekocytes (dim area could be blasts)
- 49
- 56 – natural killers
- 57
- 71 – Adult T-ALL and Mantle cell lymphoma
- 81
- 117
- 123
- 138
- 200
- HLA-DR
- FMC7
stem cells CD34+, CD31-, CD117
all leukocyte groups CD45+
Granulocyte CD45+, CD11b, CD15+, CD24+, CD114+, CD182+[17]
Monocyte CD4, CD45+, CD14+, CD114+, CD11a, CD11b, CD91+,[17] CD16+[18]
T lymphocyte CD45+, CD3+
T helper cell CD45+, CD3+, CD4+
T regulatory cell CD4, CD25, FOXP3 (a transcription factor)
Cytotoxic T cell CD45+, CD3+, CD8+
B lymphocyte CD45+, CD19+, CD20+, CD24+, CD38, CD22
Thrombocyte CD45+, CD61+
Natural killer cell CD16+, CD56+, CD3-, CD31, CD30, CD38
-from wikipedia